PurposeExpresses red fluorescent calcium sensor in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||125248||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3955
- Total vector size (bp) 5368
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Alt namejRGECO1a RS-1 EF-4
SpeciesR. norvegicus (rat), G. gallus (chicken); Discosoma sp. (Sea anemone)
Insert Size (bp)1413
- Promoter CMV
/ Fusion Protein
- NES signal
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV
- 3′ sequencing primer SV40p (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byAddgene #61563
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
fRGECO2 is the W44Y, D432A variant of the jRGECO1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGP-CMV-NEW-fRGECO2 was a gift from Katalin Torok (Addgene plasmid # 125248 ; http://n2t.net/addgene:125248 ; RRID:Addgene_125248)
For your References section:The kinetic mechanisms of fast-decay red-fluorescent genetically encoded calcium indicators. Kerruth S, Coates C, Durst CD, Oertner TG, Torok K. J Biol Chem. 2019 Mar 15;294(11):3934-3946. doi: 10.1074/jbc.RA118.004543. Epub 2019 Jan 16. 10.1074/jbc.RA118.004543 PubMed 30651353