Skip to main content
Addgene
Open Menu

pRS1422
(Plasmid #12548)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12548 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 12548-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 12548-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pIRESNeo
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5255
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Methylpurine DNA glycosylase
  • Alt name
    MPG
  • Alt name
    AAG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • GenBank ID
    NM_002434
  • Entrez Gene
    MPG (a.k.a. AAG, ADPG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mpg cDNA originally obtained from Leona Samson, MIT.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS1422 was a gift from Robert Sobol (Addgene plasmid # 12548 ; http://n2t.net/addgene:12548 ; RRID:Addgene_12548)

  • For your References section:

    The role of base excision repair in the sensitivity and resistance to temozolomide-mediated cell death. Trivedi RN, Almeida KH, Fornsaglio JL, Schamus S, Sobol RW. Cancer Res. 2005 Jul 15. 65(14):6394-400. 10.1158/0008-5472.CAN-05-0715 PubMed 16024643
Related items:
Commonly requested with: