mCNMG
(Plasmid
#1255)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1255 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS316
- Backbone size w/o insert (bp) 5276
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSUP35 NM-domain
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1664
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MutationFusion between the NM-domain of SUP35 and sGFP
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GenBank IDAY553990
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Entrez GeneSUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
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Tag
/ Fusion Protein
- sGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Sac1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCNMG was a gift from Susan Lindquist (Addgene plasmid # 1255 ; http://n2t.net/addgene:1255 ; RRID:Addgene_1255) -
For your References section:
Molecular population genetics and evolution of a prion-like protein in Saccharomyces cerevisiae. Jensen MA, True HL, Chernoff YO, Lindquist S. Genetics 2001 Oct;159(2):527-35. PubMed 11606530
