pDEST-N2H-N1
(Plasmid
#125547)
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Purpose(Empty Backbone) Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125547 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerour group built
- Backbone size (bp) 10091
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Vector typeMammalian Expression, Bacterial Expression ; in vitro expression
- Promoter synthetic promoter containing yeast, mammalian, and in vitro expression elements
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Selectable markersLEU2
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Tag
/ Fusion Protein
- NanoLuc fragment1 attached to n-terminus of Gateway cloning site (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/530790v1.full for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-N2H-N1 was a gift from Marc Vidal (Addgene plasmid # 125547 ; http://n2t.net/addgene:125547 ; RRID:Addgene_125547) -
For your References section:
Maximizing binary interactome mapping with a minimal number of assays. Choi SG, Olivet J, Cassonnet P, Vidalain PO, Luck K, Lambourne L, Spirohn K, Lemmens I, Dos Santos M, Demeret C, Jones L, Rangarajan S, Bian W, Coutant EP, Janin YL, van der Werf S, Trepte P, Wanker EE, De Las Rivas J, Tavernier J, Twizere JC, Hao T, Hill DE, Vidal M, Calderwood MA, Jacob Y. Nat Commun. 2019 Aug 29;10(1):3907. doi: 10.1038/s41467-019-11809-2. 10.1038/s41467-019-11809-2 PubMed 31467278
