-
Purposemcherry labeled DN-KASH
-
Depositing Lab
-
Depositing OrganizationVirginia Commonwealth University
Located in the United States of America -
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 125553-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 125553-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepmcherry N1
-
Backbone manufacturerclonetech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 4900
-
Vector typeMammalian Expression
-
Selectable markersGentamicin
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSYNE1
-
Alt nameDN-KASH
-
SpeciesH. sapiens (human)
-
Insert Size (bp)216
-
MutationTruncated form: only the KASH domain of nesprin-1 is expressed to create a dominant negative
-
GenBank IDNM_182961.4
-
Entrez GeneSYNE1 (a.k.a. 8B, AMC3, AMCM, ARCA1, C6orf98, CPG2, EDMD4, KASH1, MYNE1, Nesp1, SCAR8, dJ45H2.2)
- Promoter CMV
-
Tag
/ Fusion Protein
- mcherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV forward
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byThe KASH-1 sequence was synthetically produced by GeneArt.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 125553-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 125553-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
mcherry-DN KASH was a gift from Daniel Conway (Addgene plasmid # 125553 ; http://n2t.net/addgene:125553 ; RRID:Addgene_125553) -
For your References section:
Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells. Mayer CR, Arsenovic PT, Bathula K, Denis KB, Conway DE. Cell Mol Bioeng. 2019 Jul 11;12(4):289-300. doi: 10.1007/s12195-019-00579-y. eCollection 2019 Aug. 10.1007/s12195-019-00579-y PubMed 31719915
