|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12556||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2800
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameHaemophilus influenzae heat shock locus U
Alt nameH. influenzae HslU
Insert Size (bp)1400
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byOriginal insert cloned from two plasmids of the H. influenzae genomic sequencing project obtained from ATCC
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This expression plasmid was constructed from expression plasmid for HslUV complex by deleting coding sequence for HslV and inter-gene spacer
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HI.HslU.RSETB.fl.wt was a gift from David McKay (Addgene plasmid # 12556 ; http://n2t.net/addgene:12556 ; RRID:Addgene_12556)
For your References section:Structure of Haemophilus influenzae HslU protein in crystals with one-dimensional disorder twinning. Trame CB, McKay DB. Acta Crystallogr D Biol Crystallogr. 2001 Aug . 57(Pt 8):1079-90. 10.1107/S0907444901007673 PubMed 11468391