pNIC28-BpGmhA
(Plasmid
#125589)
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PurposeOverexpression plasmid for production of GmhA from Burkholderia pseudomallei
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepNIC28
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Backbone manufacturerSGC Oxford
- Backbone size w/o insert (bp) 7284
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBpGmhA
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SpeciesBurkholderia pseudomallei
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Insert Size (bp)600
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Entrez GenegmhA (a.k.a. BPSL2795)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7for
- 3′ sequencing primer T7rev; gctagttattgctcagcgg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNIC28-BpGmhA was a gift from Nicholas Harmer (Addgene plasmid # 125589 ; http://n2t.net/addgene:125589 ; RRID:Addgene_125589) -
For your References section:
The structure of sedoheptulose-7-phosphate isomerase from Burkholderia pseudomallei reveals a zinc binding site at the heart of the active site. Harmer NJ. J Mol Biol. 2010 Jul 16;400(3):379-92. doi: 10.1016/j.jmb.2010.04.058. Epub 2010 May 4. 10.1016/j.jmb.2010.04.058 PubMed 20447408
