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pPPAT-11AA-2XStrep
(Plasmid #125680)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 125680 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 7065
  • Modifications to backbone
    addition of 2X Strep-tag II by restriction cloning at the end of the MCS
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PPAT
  • Alt name
    phosphoribosyl pyrophosphate amidotransferase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1521
  • Mutation
    the first eleven residues have been removed
  • GenBank ID
  • Entrez Gene
    PPAT (a.k.a. ATASE, GPAT, PRAT)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2X Strep-Tag II (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer GAC ATC CAC TTT GCC TTT CT
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPPAT-11AA-2XStrep was a gift from Stephen Benkovic (Addgene plasmid # 125680 ; http://n2t.net/addgene:125680 ; RRID:Addgene_125680)
  • For your References section:

    Mapping Post-Translational Modifications of de Novo Purine Biosynthetic Enzymes: Implications for Pathway Regulation. Liu C, Knudsen GM, Pedley AM, He J, Johnson JL, Yaron TM, Cantley LC, Benkovic SJ. J Proteome Res. 2019 May 3;18(5):2078-2087. doi: 10.1021/acs.jproteome.8b00969. Epub 2019 Apr 18. 10.1021/acs.jproteome.8b00969 PubMed 30964683