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pBabe HAII RLF CAAX
(Plasmid #12592)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12592 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe HAII
  • Backbone size w/o insert (bp) 5137
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RLF-CAAX
  • Alt name
    RLF
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2472
  • Entrez Gene
    Rgl2 (a.k.a. KE1.5, Rab2l, Rgt2, Rlf)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI? See author map (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe HAII RLF CAAX was a gift from Channing Der (Addgene plasmid # 12592 ; http://n2t.net/addgene:12592 ; RRID:Addgene_12592)
  • For your References section:

    Oncogenic Ras blocks anoikis by activation of a novel effector pathway independent of phosphatidylinositol 3-kinase. McFall A, Ulku A, Lambert QT, Kusa A, Rogers-Graham K, Der CJ. Mol Cell Biol. 2001 Aug . 21(16):5488-99. 10.1128/MCB.21.16.5488-5499.2001 PubMed 11463831