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(Plasmid #126072)


Item Catalog # Description Quantity Price (USD)
Plasmid 126072 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Kabin Xie
  • Modifications to backbone
    Replaced hygromycin selection gene (HPTII) with BAR gene for use in maize.
  • Vector type
    Plant Expression, CRISPR
  • Promoter 35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 for gRNA/PTG
  • Selectable markers
    Basta ; BAR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pRGEB32, originally constructed by Kabin Xie from Yinong Yang's lab, was obtained from Addgene (plasmid # 63142).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector has been redesigned for use in Zea mays. Published protocols for using pRGEB32 should be used for this vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRGEB32-BAR was a gift from Charles Hunter (Addgene plasmid # 126072 ; ; RRID:Addgene_126072)
  • For your References section:

    CRISPR/Cas9 Targeted Mutagenesis for Functional Genetics in Maize. Hunter CT. Plants (Basel). 2021 Apr 8;10(4). pii: plants10040723. doi: 10.3390/plants10040723. 10.3390/plants10040723 PubMed 33917747