pET-21a(+)-DR RecR(K23A/R27A)
(Plasmid #12619)

• Depositing Lab: SeWon Suh
• Publication: Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Backbone

• Vector backbone: pET21a(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5443
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Deinococcus radiodurans RecR
• Alt name: DR RecR
• Species: Deinococcus radiodurans
• Insert Size (bp): 660
• Mutation: changed Lysine 23 to Alanine, and Arginine 27 to Alanine.
• GenBank ID: AE000513
• Entrez Gene: recR (a.k.a. DR_0198)
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry