Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pET-21a(+)-HP RecR
(Plasmid #12620)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12620 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Helicobacter pylori RecR
  • Alt name
    HP RecR
  • Species
    Helicobacter pylori
  • Insert Size (bp)
    579
  • GenBank ID
    NC_000915
  • Entrez Gene
    recR (a.k.a. HP0925)
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-21a(+)-HP RecR was a gift from SeWon Suh (Addgene plasmid # 12620 ; http://n2t.net/addgene:12620 ; RRID:Addgene_12620)
  • For your References section:

    Ring-shaped architecture of RecR: implications for its role in homologous recombinational DNA repair. Lee BI, Kim KH, Park SJ, Eom SH, Song HK, Suh SW. EMBO J. 2004 May 19. 23(10):2029-38. 10.1038/sj.emboj.7600222 PubMed 15116069