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pRSFDuet-v2 RsTAL At4CL
(Plasmid #126524)


Item Catalog # Description Quantity Price (USD)
Plasmid 126524 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3593
  • Total vector size (bp) 7026
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    tyrosine ammonia lyase
  • Alt name
  • Species
    Rhodobacter sphaeroides
  • Insert Size (bp)
  • GenBank ID
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    p-coumaroyl-CoA ligase
  • Alt name
  • Alt name
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    4CL1 (a.k.a. AT1G51680, 4-COUMARATE:COA LIGASE, 4-COUMARATE:COA LIGASE 1, 4-coumarate:CoA ligase 1, 4CL.1, ARABIDOPSIS THALIANA 4-COUMARATE:COA LIGASE 1, AT4CL1, F19C24.11, F19C24_11)
  • Promoter T7

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDuet-v2 RsTAL At4CL was a gift from Andrew Woolley (Addgene plasmid # 126524 ; ; RRID:Addgene_126524)
  • For your References section:

    Detection of Incorporation of p-Coumaric Acid into Photoactive Yellow Protein Variants in Vivo. Brechun KE, Zhen D, Jaikaran A, Borisenko V, Kumauchi M, Hoff WD, Arndt KM, Woolley GA. Biochemistry. 2019 Jun 11;58(23):2682-2694. doi: 10.1021/acs.biochem.9b00279. Epub 2019 May 30. 10.1021/acs.biochem.9b00279 PubMed 31117389