GFP-RNF2
(Plasmid #126544)

• Purpose: Expresses GFP-tagged RNF2 in mammalian cells
• Depositing Lab: Michele Pagano
• Publication: Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10.7554/eLife.38771.

Backbone

• Vector backbone: pBABE
• Backbone size w/o insert (bp): 5169
• Total vector size (bp): 6951
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RING1B
• Species: H. sapiens (human)
• Insert Size (bp): 1012
• GenBank ID: NP_009143.1
• Entrez Gene: RNF2 (a.k.a. BAP-1, BAP1, DING, HIPI3, LUSYAM, RING1B, RING2)
• Promoter: LTR
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry