GFP-RNF51
(Plasmid #126546)

• Purpose: Expresses GFP-tagged RNF51 in mammalian cells
• Depositing Lab: Michele Pagano
• Publication: Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10.7554/eLife.38771.

Backbone

• Vector backbone: pBABE
• Backbone size w/o insert (bp): 5169
• Total vector size (bp): 6147
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RNF51
• Alt name: PCGF4
• Species: H. sapiens (human)
• Insert Size (bp): 1035
• GenBank ID: NP_005171.4
• Entrez Gene: BMI1 (a.k.a. FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1)
• Promoter: LTR
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry