FLAG-SENP2 CCm
(Plasmid #126594)

• Purpose: Expresses siRNA resistant SENP2 (coiled-coil deletion) in mammalian cells, Dox inducible in TetR cell lines
• Depositing Lab: Joanna Morris
• Publication: Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. doi: 10.1101/gad.321125.118. Epub 2019 Feb 22.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5137
• Total vector size (bp): 6853
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SENP2
• Species: H. sapiens (human)
• Insert Size (bp): 1716
• Mutation: Deletion of amino acids 203-228 and siRNA resistant to GAGGAGAUAUUCAGACAUU
• GenBank ID: 59343
• Entrez Gene: SENP2 (a.k.a. AXAM2, SMT3IP2)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (unknown if destroyed)
• 3′ cloning site: Eco RV (unknown if destroyed)
• 5′ sequencing primer: CMV_F
• 3′ sequencing primer: bGH_R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Deletion of the coiled-coil domain between amino acids 203-228