pET-21a(+) SurE D8A
(Plasmid #12685)

• Depositing Lab: SeWon Suh
• Publication: Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Backbone

• Vector backbone: pET-21a(+)
• Backbone size w/o insert (bp): 5443
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SurE
• Alt name: TM1662
• Insert Size (bp): 741
• Mutation: changed Aspartate 8 to Alanine
• Entrez Gene: TM1662
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry