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pMS17
(Plasmid #127088)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127088 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSET152
  • Backbone size w/o insert (bp) 4460
  • Total vector size (bp) 5894
  • Vector type
    Streptomyce integration vector derived from phiC31 int/attP

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    tcp830
  • Alt name
    Tetracycline inducible promoter
  • Species
    Synthetic
  • Insert Size (bp)
    69

Gene/Insert 2

  • Gene/Insert name
    TetRiS
  • Alt name
    Tetracycline repressor (TetR) adapted for use in Streptomyces
  • Species
    E. coli
  • Insert Size (bp)
    634

Gene/Insert 3

  • Gene/Insert name
    terminator mmrt
  • Species
    Streptomyces
  • Insert Size (bp)
    144

Gene/Insert 4

  • Gene/Insert name
    Terminator fdt
  • Species
    E. coli
  • Insert Size (bp)
    426

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For research using this plasmid, please acknowledge the depositing laboratory and associated citation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS17 was a gift from Maggie Smith (Addgene plasmid # 127088 ; http://n2t.net/addgene:127088 ; RRID:Addgene_127088)
  • For your References section:

    Natural and synthetic tetracycline-inducible promoters for use in the antibiotic-producing bacteria Streptomyces. Rodriguez-Garcia A, Combes P, Perez-Redondo R, Smith MC, Smith MC. Nucleic Acids Res. 2005 May 25;33(9):e87. doi: 10.1093/nar/gni086. 10.1093/nar/gni086 PubMed 15917435