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(Plasmid #12723)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 12723 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pcDNA 3.1D V5-His-TOPO
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5514
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
    ribose 2'-Omethyltransferase
  • Species
    Vaccinia Virus
  • Insert Size (bp)
  • Entrez Gene
    J3R (a.k.a. VACWR095)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Universal T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the Vaccinia kit page ( ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DCT.VV.Orf35 was a gift from Jack Bennink & Jon Yewdell (Addgene plasmid # 12723 ; ; RRID:Addgene_12723)
  • For your References section:

    Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines. Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW. J Exp Med. 2005 January 3; 201(1): 95–104. 10.1084/jem.20041912 PubMed 15623576