Full human ELL2
(Plasmid #127253)

• Purpose: Expresses full-length human ELL2 in mammalian cells
• Depositing Lab: Christine Milcarek
• Publication: Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009 Sep 13.

Backbone

• Vector backbone: pEF4B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5800
• Vector type: Mammalian Expression ; Other
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ELL2
• Alt name: CM514
• Alt name: IMAGE Clone4822236
• Species: H. sapiens (human)
• Insert Size (bp): 1900
• Entrez Gene: ELL2 (a.k.a. MRCCAT1)
• Promoter: pEF1a
• Tag / Fusion Protein:
  • His6X (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: IMAGE Clone4822236

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

6aa bw His tag and AUG of ELL2 in frame, unstable in 293T