Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

6N31
(Plasmid #127293)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127293 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid #62304)
  • Vector type
    Baculovirus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WDR12
  • Alt name
    6N31
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    999
  • Entrez Gene
    WDR12 (a.k.a. YTM1)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: WDR12:JMC061-H05:C224099. SGC or PDB link: http://www.thesgc.org/structures/6N31/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6N31 was a gift from Cheryl Arrowsmith (Addgene plasmid # 127293 ; http://n2t.net/addgene:127293 ; RRID:Addgene_127293)