PurposeBacterial expression for structure determination. May not contain entire coding region of gene
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||127295||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerSGC (Addgene Plasmid # 26092)
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Insert Size (bp)390
- Promoter T7
/ Fusion Protein
- 6xHis-TEV (N terminal on backbone)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID:TcCLB.506755.120:MAC0FE-B05:C243137. SGC or PDB link: http://www.thesgc.org/structures/6NEY/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:6NEY was a gift from Cheryl Arrowsmith (Addgene plasmid # 127295 ; http://n2t.net/addgene:127295 ; RRID:Addgene_127295)