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Nanog-t2A-mCherry
(Plasmid #127541)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127541 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.3-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6149
  • Total vector size (bp) 8149
  • Modifications to backbone
    Inserted T2a-mCherry at 3' terminal of MCS.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nanog
  • Alt name
    ENK
  • Alt name
    ecat4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2000
  • Mutation
    Codes for the last 110 a.a of Nanog
  • GenBank ID
    NM_001289830
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, ENK, ecat4)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer TTGGTCACCTTCAGCTTGG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid codes for the activation domain of mouse Nanog (last 110 a.a).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nanog-t2A-mCherry was a gift from Jennifer Mitchell (Addgene plasmid # 127541 ; http://n2t.net/addgene:127541 ; RRID:Addgene_127541)
  • For your References section:

    KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control. Dhaliwal NK, Abatti LE, Mitchell JA. Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119. 10.1101/gad.324319.119 PubMed 31221664