pCAG-mEGFP-CaMKIIa (T305D/T306D)
(Plasmid
#127611)
-
PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)
-
Depositing Lab
-
Depositing OrganizationMax Planck Florida Institute
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127611 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 127611-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 127611-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneCustomized Vector
- Total vector size (bp) 6921
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namecalcium/calmodulin-dependent protein kinase II alpha
-
Alt nameCaMKIIa
-
Alt nameCamk2a
-
SpeciesR. norvegicus (rat)
-
Insert Size (bp)1434
-
Mutationchanged Threonine 305/306 to Alanine
-
GenBank IDNM_012920.1
-
Entrez GeneCamk2a (a.k.a. PK2CDD, PKCCD)
- Promoter pCAG
-
Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GCCTCTGCTAACCATGTTC, AGAGCAGCAGGCATGGTTT
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 127611-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 127611-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCAG-mEGFP-CaMKIIa (T305D/T306D) was a gift from Ryohei Yasuda (Addgene plasmid # 127611 ; http://n2t.net/addgene:127611 ; RRID:Addgene_127611) -
For your References section:
Mechanisms of Ca(2+)/calmodulin-dependent kinase II activation in single dendritic spines. Chang JY, Nakahata Y, Hayano Y, Yasuda R. Nat Commun. 2019 Jun 25;10(1):2784. doi: 10.1038/s41467-019-10694-z. 10.1038/s41467-019-10694-z PubMed 31239443
Map uploaded by the depositor.
