pTK-EdnrbE2-Sox10-FLAG-tev-AVI
(Plasmid
#127775)
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PurposeModified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127775 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTK
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Vector typeEnhancer reporter construct
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesox10
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SpeciesG. gallus (chicken)
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Entrez GeneSOX10 (a.k.a. SOX-10)
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Tag
/ Fusion Protein
- Avi (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTK-EdnrbE2-Sox10-FLAG-tev-AVI was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 127775 ; http://n2t.net/addgene:127775 ; RRID:Addgene_127775) -
For your References section:
Early chromatin shaping predetermines multipotent vagal neural crest into neural, neuronal and mesenchymal lineages. Ling ITC, Sauka-Spengler T. Nat Cell Biol. 2019 Dec;21(12):1504-1517. doi: 10.1038/s41556-019-0428-9. Epub 2019 Dec 2. 10.1038/s41556-019-0428-9 PubMed 31792380
