pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A
(Plasmid #127790)

• Purpose: Expresses a CtCHAD mutant protein in E. coli cells
• Depositing Lab: Michael Hothorn
• Publication: Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii: 2/3/e201900385. doi: 10.26508/lsa.201900385. Print 2019 Jun.

Backbone

• Vector backbone: pMH-HT
• Backbone manufacturer: Michael Hothorn
• Backbone size w/o insert (bp): 5311
• Total vector size (bp): 6262
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CtCHAD
• Alt name: Q8KE09
• Species: Chlorobium tepidum
• Insert Size (bp): 951
• Mutation: Residues 208-522 (CHAD domain), mutations in His253, Arg256, Arg260, Arg296, Arg418 and Tyr419 to Ala
• Entrez Gene: CT0884 (a.k.a. CT0884)
• Tag / Fusion Protein:
  • 6xHis tag + TEV cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: TGCGTCCGGCGTAGAGGATCGAGATCT
• 3′ sequencing primer: CAGCTTCCTTTCGGGCTTTGTTAGCAG

Resource Information

• Supplemental Documents:
  • pMH-HT_CtCHAD-R253A-R256A-R260A-R296A-R418A-Y419A.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry