pCDNA6-LRRC26-V5-His
(Plasmid
#127817)
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PurposeExpression of human LRRC26 in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversity of Texas MD Anderson Cancer Center
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127817 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 127817-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 127817-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCDNA6
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLRRC26
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Alt nameBK channel auxiliary gamma subunit 1
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SpeciesH. sapiens (human)
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Entrez GeneLRRC26 (a.k.a. CAPC, bA350O14.10)
- Promoter CMV
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Tag
/ Fusion Protein
- V5, 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 127817-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 127817-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA6-LRRC26-V5-His was a gift from Jiusheng Yan (Addgene plasmid # 127817 ; http://n2t.net/addgene:127817 ; RRID:Addgene_127817) -
For your References section:
Molecular basis for differential modulation of BK channel voltage-dependent gating by auxiliary gamma subunits. Li Q, Fan F, Kwak HR, Yan J. J Gen Physiol. 2015 Jun;145(6):543-54. doi: 10.1085/jgp.201511356. 10.1085/jgp.201511356 PubMed 26009545
