CFP-CitA6-130-Venus
(Plasmid
#128016)
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PurposeUnmodified FLIP CitA6-130 sensor
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128016 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRSET
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsConstruction and testing of plasmid was done in BL21.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCitA
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SpeciesKlebsiella pneumoniae
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Mutationsee Depositor Comments
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene identified the following mutations: S323N, A375E, R556G
These are not expected to impact plasmid function
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CFP-CitA6-130-Venus was a gift from Uwe Sauer (Addgene plasmid # 128016 ; http://n2t.net/addgene:128016 ; RRID:Addgene_128016) -
For your References section:
Engineering genetically encoded nanosensors for real-time in vivo measurements of citrate concentrations. Ewald JC, Reich S, Baumann S, Frommer WB, Zamboni N. PLoS One. 2011;6(12):e28245. doi: 10.1371/journal.pone.0028245. Epub 2011 Dec 2. 10.1371/journal.pone.0028245 PubMed 22164251
