Skip to main content
Addgene
Open Menu

CFP-CitA6-130-Venus
(Plasmid #128016)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128016 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 128016-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 128016-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pRSET

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Construction and testing of plasmid was done in BL21.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CitA
  • Species
    Klebsiella pneumoniae
  • Mutation
    see Depositor Comments

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene identified the following mutations: S323N, A375E, R556G
These are not expected to impact plasmid function

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CFP-CitA6-130-Venus was a gift from Uwe Sauer (Addgene plasmid # 128016 ; http://n2t.net/addgene:128016 ; RRID:Addgene_128016)

  • For your References section:

    Engineering genetically encoded nanosensors for real-time in vivo measurements of citrate concentrations. Ewald JC, Reich S, Baumann S, Frommer WB, Zamboni N. PLoS One. 2011;6(12):e28245. doi: 10.1371/journal.pone.0028245. Epub 2011 Dec 2. 10.1371/journal.pone.0028245 PubMed 22164251
Related items:
Commonly requested with: