pSB4C5_pBen-BenR
(Plasmid #128127)

• Purpose: Expressing BenR transcription factor gene under control of benzoate responsive promoter (pBen) in a feedback loop.
• Depositing Lab: Jean-Loup Faulon
• Publication: Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.

Backbone

• Vector backbone: pSB4C5 (iGEM registry)
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 7 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: BenR
• Species: Pseudomonas putida
• Insert Size (bp): 957
• Promoter: pBen

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI, golden gate (unknown if destroyed)
• 3′ cloning site: BsaI, golden gate (unknown if destroyed)
• 5′ sequencing primer: ATACGCAAGGCGACAA

Resource Information

• Supplemental Documents:
  • psb4c5_pben-benr.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a single C nucleotide insertion in the pBen promoter sequence. It is not known if this mutation affects plasmid function.