pBEAST_J23101-HipO
(Plasmid #128133)

• Purpose: The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032
• Depositing Lab: Jean-Loup Faulon
• Publication: Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.

Backbone

• Vector backbone: pBEAST
• Backbone size w/o insert (bp): 2987
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HipO
• Alt name: Hippurate hydrolase (EC: 3.5.1.32), UniProtKB - P45493
• Species: From Campylobacter jejuni, codon optimized for E. coli
• Insert Size (bp): 1152
• Promoter: J23101

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI, golden gate (unknown if destroyed)
• 3′ cloning site: BsaI, golden gate (unknown if destroyed)
• 5′ sequencing primer: ggcaactgaaacgattcg
• 3′ sequencing primer: ttgatgcctggcaccaac

Resource Information

• Supplemental Documents:
  • pbeast_j23101-hipo.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Similar to our previously deposited sequence into addgene pBEST-HipO (Plasmid #114599), but with a different promoter and modified backbone.