LBR EGFP
(Plasmid #128150)

• Purpose: Expression of lamin B receptor
• Depositing Lab: Jennifer Lippincott-Schwartz
• Publication: Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-206.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: lamin B receptor
• Species: H. sapiens (human)
• Entrez Gene: LBR (a.k.a. C14SR, DHCR14B, LMN2R, PHA, PHASK, TDRD18)

Cloning Information

• Cloning method: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The LBR-GFP fusion used in this study includes the first 238 amino acids of human LBR (Ye and Worman, 1994) fused to the F64L, S65T, H231L variant of the GFP with a four-amino acid spacer (PVAT) in the expression vector pEGFP-N1 (CLONTECH Laboratories, Palo Alto, CA) and was generated by standard procedures (Sambrook et al., 1989).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry