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RFP Dynamin2 K44A
(Plasmid #128153)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 128153 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dynamin 2
  • Species
    R. norvegicus (rat)
  • Mutation
    K44A
  • Entrez Gene
    Dnm2 (a.k.a. DYIIAAB)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains D406G, V664A, H692R, and Q758R mutations in Dynamin2 compared to the NCBI reference sequence XP_006242670.1. These mutations are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RFP Dynamin2 K44A was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 128153 ; http://n2t.net/addgene:128153 ; RRID:Addgene_128153)
  • For your References section:

    Dynamin 2 orchestrates the global actomyosin cytoskeleton for epithelial maintenance and apical constriction. Chua J, Rikhy R, Lippincott-Schwartz J. Proc Natl Acad Sci U S A. 2009 Nov 30. ():. 10.1073/pnas.0909812106 PubMed 19948954