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pCMS-dsRed2-WT LMNA-GFP minigene
(Plasmid #128230)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 128230 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMS-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lamin A
  • Alt name
    LMNA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    850
  • Entrez Gene
    LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GFP (C terminal on backbone)
    • SV40-driven dsRed2 to identify transfected cells.

Cloning Information

Resource Information

Depositor Comments

WT lamin A splicing reporter with short-lived GFP downstream LMNA exon 12 (CMV-driven) + SV40-driven dsRed2 to identified transfected cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMS-dsRed2-WT LMNA-GFP minigene was a gift from Tom Misteli (Addgene plasmid # 128230 ; http://n2t.net/addgene:128230 ; RRID:Addgene_128230)
  • For your References section:

    Interplay of primary sequence, position and secondary RNA structure determines alternative splicing of LMNA in a pre-mature aging syndrome. Shilo A, Tosto FA, Rausch JW, Le Grice SFJ, Misteli T. Nucleic Acids Res. 2019 Apr 22. pii: 5475939. doi: 10.1093/nar/gkz259. 10.1093/nar/gkz259 PubMed 31006814