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pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry
(Plasmid #128327)


Item Catalog # Description Quantity Price (USD)
Plasmid 128327 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Modifications to backbone
    Please refer to the paper.
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    H2B mCherry
  • Species
    H. sapiens (human)

Gene/Insert 2

  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    H2B-mCherry from Robert Benezra (Addgene plasmid #20972)
  • Article Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCIneoFH-YAP1 was digested with NotI, filled in, and cut with NheI. The isolated fragment was ligated into NheI/SmaI sites of pLL3.7 K122 to generate pLL3.7 K122 FH-YAP1. NcoI/BglII from pEYFP-C1 (Clontech Laboratories) was ligated into NcoI/BamHI of pGL3 to replace luciferase with YFP and to generate YFP reporter. BamHI/Sall and Sall/HindIII fragments from 8xGT-IIC-d51LucII luciferase reporter (a gift from Hiroshi Sasaki; RIKEN RDB08067) were ligated into BglII/HindIII sites of the YFP reporter to generate pTEAD-responsive-promoter YFP, which was digested with NotI and partially digested with HindIII. The resulting fragment and HindIII/XbaI fragment from H2B-mCherry (a gift from Robert Benezra; Addgene plasmid #20972) were ligated into NotI/XbaI sites of pLL3.7 K122 FH-YAP1 and pLL3.7 K122 to obtain pLL3.7 K122 FH-YAP1-ires-GFP-TEAD-responsive-promoter-H2B-mCherry reporter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry was a gift from Yutaka Hata (Addgene plasmid # 128327 ; ; RRID:Addgene_128327)
  • For your References section:

    Novel YAP1 Activator, Identified by Transcription-Based Functional Screen, Limits Multiple Myeloma Growth. Maruyama J, Inami K, Michishita F, Jiang X, Iwasa H, Nakagawa K, Ishigami-Yuasa M, Kagechika H, Miyamura N, Hirayama J, Nishina H, Nogawa D, Yamamoto K, Hata Y. Mol Cancer Res. 2018 Feb;16(2):197-211. doi: 10.1158/1541-7786.MCR-17-0382. Epub 2017 Oct 23. 10.1158/1541-7786.MCR-17-0382 PubMed 29061667