pGEX-UbcH7(C86K)-10aa-linker-delta 1-29, 117-160 and 412-496 Bactrocera parkin
(Plasmid #128384)

• Purpose: expresses protein UbcH7 (C86K) fused to Bactrocera parkin with residues 1-29, 117-160 and 412-496 deleted
• Depositing Lab: Kalle Gehring
• Publication: Sauve et al Nat Struct Mol Biol. 2018 Jul;25(7):623-630. doi: 10.1038/s41594-018-0088-7. Epub 2018 Jul 2.

Backbone

• Vector backbone: pGEX-6P1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 5221
• Total vector size (bp): 6727
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: lower temperature to 16C for protein expression in BL21
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UbcH7
• Species: H. sapiens (human)
• Insert Size (bp): 1506
• Mutation: mutation C86K in UbcH7 gene
• Entrez Gene: UBE2L3 (a.k.a. E2-F1, L-UBC, UBCH7, UbcM4)
• Promoter: lac promotor
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • Bactrocera dorsalis parkin deleted residues 1 to 29, 117 to 160 and 412 to 496 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGgatccATGGCGGCCAGCAGGAGGCTG
• 3′ sequencing primer: gccaccgccggatccctggaagtacaggttttcaccgccGTCCACAGGTCGCTTTTCCCC

Resource Information

• Supplemental Documents:
  • pPA126.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry