pDONR_P4-P1R:PER36modp
(Plasmid #128431)

• Purpose: Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).
• Depositing Lab: George Haughn
• Publication: McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 10.1007/s11103-019-00909-8.

Backbone

• Vector backbone: pDONR_P4-P1R
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5892
• Total vector size (bp): 5278
• Vector type: Gateway promoter entry clone

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For selection use 50 ug/ml Kanamycin
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PEROXISADE36
• Alt name: PER36
• Alt name: PRX36
• Alt name: At3g50990
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2635
• Mutation: Premature stop codons introduced into the Xero1 (cytosine to thymine mutation at 1,560 bp) and Xero2 (guanine to thymine mutation at 345 bp) genes in the PER36 promoter insert
• GenBank ID: BT010535
• Entrez Gene: AT3G50990 (a.k.a. AT3G50990, PER36, peroxidase 36)
• Promoter: PEROXISADE36

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: 5’-TGTAAAACGACGGCCAGT-3
• 3′ sequencing primer: 5’-GAAGCATACCGAGCTAGAGC-3’

Resource Information

• Supplemental Documents:
  • pDONR P4-P1R-PER36modp- SnapGene File
• A portion of this plasmid was derived from a plasmid made by: The PER36 promoter (2,635 bp) was originally cloned into the pDONR P4-P1R entry clone as described in Kunieda T, Shimada T, Kondo M, Nishimura M, Nishitani K, Hara-Nishimura I (2013) Spatiotemporal Secretion of PEROXIDASE36 Is Required for Seed Coat Mucilage Extrusion in Arabidopsis. Plant Cell 25:1355–1367. doi: 10.1105/tpc.113.110072

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

For use with Three-way Multi-site Gateway Cloning (Invitrogen).