pIE-TGFB2-H6
(Plasmid #128500)

• Purpose: Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.
• Depositing Lab: F Xavier Gomis-Ruth
• Publication: Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z.

Backbone

• Vector backbone: pIEx
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3843
• Total vector size (bp): 5025
• Modifications to backbone: Introduction of T after the signal peptide to introduce StuI restriction site = ATGTACAAGCTCACAGTCTTCCTGATGTTCATCGCTTTCGTCATCATCGCTGAGGCCt After AgeI restriction site; introductionn of 6x-His and stop codon = ACCGGTcatcatcaccatcaccatTGA
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TGFB2
• Alt name: Transforming growth factor beta-2 proprotein
• Alt name: Cetermin
• Alt name: Glioblastoma-derived T-cell suppressor factor
• Species: H. sapiens (human)
• Insert Size (bp): 1182
• GenBank ID: NP_001129071.1
• Entrez Gene: TGFB2 (a.k.a. G-TSF, LDS4, TGF-beta2)
• Promoter: IE1
• Tag / Fusion Protein:
  • His tag (6x) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: StuI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: AGGCCTTGTCTACCTGCAGCACACTC
• 3′ sequencing primer: ACCGGTGCTGCATTTGCAAGACTTTAC

Resource Information

• Supplemental Documents:
  • pro-TGFβ2_pIE (L21-S414).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry