pS6-TGFB2-H8
(Plasmid #128502)

• Purpose: Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.
• Depositing Lab: F Xavier Gomis-Ruth
• Publication: Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.1038/s41598-019-45712-z.

Backbone

• Vector backbone: pCMV-Sport 6
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4396
• Total vector size (bp): 5578
• Modifications to backbone: Introduction between SmaI site and L21 (tgfb2) of Kozak sequence, Igk leader and 8x-His
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TGFB2 (L21-S414)
• Alt name: Transforming growth factor beta-2 proprotein
• Alt name: Cetermin
• Alt name: Glioblastoma-derived T-cell suppressor factor
• Species: H. sapiens (human)
• Insert Size (bp): 1182
• GenBank ID: NP_001129071.1
• Entrez Gene: TGFB2 (a.k.a. G-TSF, LDS4, TGF-beta2)
• Promoter: CMV
• Tag / Fusion Protein:
  • His tag (6x) (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CAATCCCGGGGCCACCATGGAGACAGACACACTCC;TGCTATGGGTACTGCTGCTCTGGGTTCCAGGTTCCACTGGTGACCACCACCATCACCACCACCATCATCTCAGCcTgtctacctgcagca
• 3′ sequencing primer: CAATCTCGAGCTAGCTGCATTTGCAAGACTTTAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry