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pLZRS-ER-Rac1-Q61L-shortlinker
(Plasmid #128584)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 128584 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLZRS
  • Backbone manufacturer
    Addgene plasmid 21199
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Rac1
  • Species
    M. musculus (mouse)
  • Mutation
    Changed Glutamine61 to Leucine in the RAC1 moiety.
  • Entrez Gene
    Rac1 (a.k.a. D5Ertd559e)
  • Tag / Fusion Protein
    • Modified hormone binding domain of ER alpha (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLZRS-ER-Rac1-Q61L-shortlinker was a gift from Julian Downward (Addgene plasmid # 128584 ; http://n2t.net/addgene:128584 ; RRID:Addgene_128584)
  • For your References section:

    RAC1(P29S) Induces a Mesenchymal Phenotypic Switch via Serum Response Factor to Promote Melanoma Development and Therapy Resistance. Lionarons DA, Hancock DC, Rana S, East P, Moore C, Murillo MM, Carvalho J, Spencer-Dene B, Herbert E, Stamp G, Damry D, Calado DP, Rosewell I, Fritsch R, Neubig RR, Molina-Arcas M, Downward J. Cancer Cell. 2019 Jul 8;36(1):68-83.e9. doi: 10.1016/j.ccell.2019.05.015. Epub 2019 Jun 27. 10.1016/j.ccell.2019.05.015 PubMed 31257073