pDM304_YFP-IqgC_mRFP-Raf1(RBD)
(Plasmid #128698)

• Purpose: simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells
• Depositing Lab: Igor Weber
• Publication: Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;116(4):1289-1298. doi: 10.1073/pnas.1810268116. Epub 2019 Jan 8.

Backbone

• Vector backbone: pDM304
• Backbone manufacturer: Veltman et al (2009)
• Vector type: Dictyostelium discoideum expression vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: iqgC (full length)
• Species: Dictyostelium discoideum
• Mutation: none
• Tag / Fusion Protein:
  • YFP (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: Raf1 kinase (aa 50-132)
• Species: M. musculus (mouse)
• Mutation: none
• Tag / Fusion Protein:
  • mRFP (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results verified the insert of this plasmid but cannot confirm the entire backbone sequence. We recommend doing additional diagnostic tests before use. Depositor confirms plasmid functions as described in their publication.