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pDM358_DPAKa(GBD)-YFP
(Plasmid #128700)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128700 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 128700-D50 50 μg of DNA in Tris buffer $495
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DNA 128700-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pDM358
  • Backbone manufacturer
    Veltman et al (2009)
  • Vector type
    Dictyostelium discoideum expression vector
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GBD domain (aa 731-890) of DPAKa
  • Alt name
    pakA
  • Alt name
    dpak1
  • Species
    Dictyostelium discoideum
  • Mutation
    none
  • Tag / Fusion Protein
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results verified the insert of this plasmid but cannot confirm the entire backbone sequence. We recommend doing additional diagnostic tests before use. Depositor confirms plasmid functions as described in their publication.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDM358_DPAKa(GBD)-YFP was a gift from Igor Weber (Addgene plasmid # 128700 ; http://n2t.net/addgene:128700 ; RRID:Addgene_128700)

  • For your References section:

    Quantitative imaging of Rac1 activity in Dictyostelium cells with a fluorescently labelled GTPase-binding domain from DPAKa kinase. Marinovic M, Sostar M, Filic V, Antolovic V, Weber I. Histochem Cell Biol. 2016 Sep;146(3):267-79. doi: 10.1007/s00418-016-1440-9. Epub 2016 Apr 28. 10.1007/s00418-016-1440-9 PubMed 27126594
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