pDM358_DPAKa(GBD)-YFP
(Plasmid #128700)

• Purpose: expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells
• Depositing Lab: Igor Weber
• Publication: Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267-79. doi: 10.1007/s00418-016-1440-9. Epub 2016 Apr 28.

Backbone

• Vector backbone: pDM358
• Backbone manufacturer: Veltman et al (2009)
• Vector type: Dictyostelium discoideum expression vector
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GBD domain (aa 731-890) of DPAKa
• Alt name: pakA
• Alt name: dpak1
• Species: Dictyostelium discoideum
• Mutation: none
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results verified the insert of this plasmid but cannot confirm the entire backbone sequence. We recommend doing additional diagnostic tests before use. Depositor confirms plasmid functions as described in their publication.