MBP-TEV-Gly-RadA∆C46
(Plasmid
#128702)
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PurposeExpresses PhoRadA intein split at residue 126 from the N-terminal
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128702 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepMAL-c5X
- Backbone size w/o insert (bp) 5677
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRadA
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SpeciesPyrococcus horikoshii
- Promoter T7
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Tag
/ Fusion Protein
- Hisx6-MBP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MBP-TEV-Gly-RadA∆C46 was a gift from Sharon Rozovsky (Addgene plasmid # 128702 ; http://n2t.net/addgene:128702 ; RRID:Addgene_128702) -
For your References section:
Efficient generation of hydrazides in proteins by RadA split intein. Liu J, Ekanayake O, Santoleri D, Walker K, Rozovsky S. Chembiochem. 2019 Jul 2. doi: 10.1002/cbic.201900160. 10.1002/cbic.201900160 PubMed 31265209
