pARS3_MUbCAS9_MC
(Plasmid
#128767)
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Purpose(Empty Backbone) T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNA
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128767 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepARS3
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Backbone manufacturerThilmony,R.
- Backbone size (bp) 7700
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Modifications to backboneThe T-DNA portion of the vector incorporates MCS for cloning gRNAs, a transformation booster sequence (TBS), a plant-codon-optimized hemagglutinin (HA)- tagged nuclear-localized Cas9 gene (Cas9-HA-N7), driven by the Zea mays UBQ10 promoter and containing a ribosomal-binding site (RBS), and OCS terminator.
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Vector typePlant Expression
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- HA
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pARS3_MUbCAS9_MC was a gift from Zachary Nimchuk (Addgene plasmid # 128767 ; http://n2t.net/addgene:128767 ; RRID:Addgene_128767) -
For your References section:
Type-B response regulators of rice play key roles in growth, development, and cytokinin signaling. Worthen JM, Yamburenko MV, Lim J, Nimchuk ZL, Kieber JJ, Schaller GE. Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870. 10.1242/dev.174870 PubMed 31160418
Map uploaded by the depositor.
