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pGEX-PP-Spastin(87-616)DeltaExon4
(Plasmid #128794)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 128794 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX
  • Backbone size w/o insert (bp) 4984
  • Total vector size (bp) 6481
  • Vector type
    Bacterial Expression ; `

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spastin
  • Alt name
    SPAST
  • Alt name
    SPG4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1497
  • Mutation
    residues 87-616 without exon 4.
  • GenBank ID
    NC_000002.12
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter T7
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site BanH1 (not destroyed)
  • 5′ sequencing primer GCAAGCCACGTTTGG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-PP-Spastin(87-616)DeltaExon4 was a gift from Chris Hill (Addgene plasmid # 128794 ; http://n2t.net/addgene:128794 ; RRID:Addgene_128794)
  • For your References section:

    Structure of spastin bound to a glutamate-rich peptide implies a hand-over-hand mechanism of substrate translocation. Han H, Schubert HL, McCullough J, Monroe N, Purdy MD, Yeager M, Sundquist WI, Hill CP. J Biol Chem. 2019 Nov 25. pii: AC119.009890. doi: 10.1074/jbc.AC119.009890. 10.1074/jbc.AC119.009890 PubMed 31767681