Skylan-S-(GGGGS)x3-β-Actin
(Plasmid #128938)

• Purpose: fluorescnece reporter of β-Actin with Skylan-S. Express in mammalian cells
• Depositing Lab: Shimon Weiss
• Publication: Yi et al Biomed Opt Express. 2019 Apr 16;10(5):2430-2445. doi: 10.1364/BOE.10.002430. eCollection 2019 May 1.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5446
• Total vector size (bp): 6100
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Skylan-S
• Insert Size (bp): 676
• Tag / Fusion Protein:
  • β-actin

Resource Information

• Supplemental Documents:
  • Skylan-S sequence
• A portion of this plasmid was derived from a plasmid made by: We received Skylan-S encoding plasmid from Dr. Pingyong Xu, and created the fusion plasmid of Skylan-S with β-actin in the lab of Dr. Shimon Weiss.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the HindIII site following the GGGGS linker depicted in the depositor's plasmid map is not unique. There are 3 HIndIII cut sites present in this plasmid (bp891, 1193, and 1656 in the Addgene NGS Result).

The article that introduces Skylan protein family:
Zhang, X., Chen, X., Zeng, Z., Zhang, M., Sun, Y., Xi, P., ... & Xu, P. (2015). Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI). ACS nano, 9(3), 2659-2667. (https://www.ncbi.nlm.nih.gov/pubmed/25695314)

The article that created and used Skylan-S-(GGGGS)x3-β-actin for super-resolution:
Xiyu Yi, Sungho Son, Ryoko Ando, Atsushi Miyawaki, and Shimon Weiss, "Moments reconstruction and local dynamic range compression of high order superresolution optical fluctuation imaging," Biomed. Opt. Express 10, 2430-2445 (2019)