pTAMACHis6_PiuD
(Plasmid #128953)

• Purpose: expresses PiuD in E.coli
• Depositing Lab: James Naismith
• Publication: Luscher et al Antimicrob Agents Chemother. 2018 May 25;62(6). pii: AAC.00097-18. doi: 10.1128/AAC.00097-18. Print 2018 Jun.

Backbone

• Vector backbone: pTAMACHIS6
• Backbone size w/o insert (bp): 3725
• Total vector size (bp): 5866
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: protein expression in C43(DE3) strains
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Putative outer membrane receptor for iron transport (PiuD)
• Alt name: PiuD
• Species: P. aeruginosa strain 39016
• Insert Size (bp): 2193
• Mutation: Signal peptide sequence has been removed (1-105)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (C terminal on backbone)
  • TamA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspHI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pTAMACHis6 expression vector was obtained by replacing the PelB signal peptide of pEPELBCHIS (courtesy of Huanting Liu, University St Andrews) with the TamA signal peptide