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co luxE pcDNA3.1(+)
(Plasmid #129089)


Item Catalog # Description Quantity Price (USD)
Plasmid 129089 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5378
  • Total vector size (bp) 6491
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    co luxE
  • Alt name
    codon-optimized luxE
  • Species
  • Insert Size (bp)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TGACGCAAATGGGCGGTA
  • 3′ sequencing primer GAAAGGACAGTGGGAGTGGCA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    co luxE pcDNA3.1(+) was a gift from Stefan Hell (Addgene plasmid # 129089 ; ; RRID:Addgene_129089)
  • For your References section:

    Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system. Gregor C, Pape JK, Gwosch KC, Gilat T, Sahl SJ, Hell SW. Proc Natl Acad Sci U S A. 2019 Dec 2. pii: 1913616116. doi: 10.1073/pnas.1913616116. 10.1073/pnas.1913616116 PubMed 31792180