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(Plasmid #129106)


Item Catalog # Description Quantity Price (USD)
Plasmid 129106 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Adapted from pCMV_BE4max_P2A_GFP
  • Backbone size w/o insert (bp) 6049
  • Total vector size (bp) 10174
  • Modifications to backbone
    Added WPRE
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Sc++ CDS
  • Species
  • Insert Size (bp)
  • Mutation
    I365A, G366D, I367K, H369L, T373S, T374G, Q379E, T1227K
  • Promoter EF1⍺
  • Tag / Fusion Protein
    • NLS-P2A-EGFP-NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note the name and information of this plasmid have been updated to Plasmid 155011: Sc++ in the Chatterjee et al Nat Biotechnol. 2020 publication. Please see the new webpage:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSc++ was a gift from Joseph Jacobson (Addgene plasmid # 129106)