pSK260
(Plasmid #129252)

• Purpose: dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter
• Depositing Lab: Ahmad Khalil
• Publication: Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi: 10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.

Backbone

• Vector backbone: pGAN200
• Backbone manufacturer: Gregory Newby
• Total vector size (bp): 9814
• Modifications to backbone: replaced mNeonGreen reporter with yeast optimized mKate2
• Vector type: Yeast Expression
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sup35 NM yTRAP sensor reporting on mKate2
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • pSK260_NAT_HO_Sup-mKate2-43-8_SUP35NM.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the Sup35NM yTRAP sensor plasmid reporting on mKate2 for dual prion sensing. pSK260 should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin.